1-mm screen of a wiley mill Search Results


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Loading mature cortical slices with <t>fura-2</t> AM. DIC (A) and fluorescence (B) images of the lower layers of a visual cortex slice from a P10 mouse with the pial surface to the bottom right. The slice was loaded with fura-2 AM. Somata and apical dendrites from many neurons are fluorescent, and pyramidal and nonpyramidal neurons label equally. (Scale bar = 20 μm.) (C) Percentage of loaded neurons as a function of the age of the animal. Each point represents one brain slice. Loading is close to 100% at P10, but it declines to ≈70% of the neurons at P20. The line represents the best linear fit.
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Loading mature cortical slices with <t>fura-2</t> AM. DIC (A) and fluorescence (B) images of the lower layers of a visual cortex slice from a P10 mouse with the pial surface to the bottom right. The slice was loaded with fura-2 AM. Somata and apical dendrites from many neurons are fluorescent, and pyramidal and nonpyramidal neurons label equally. (Scale bar = 20 μm.) (C) Percentage of loaded neurons as a function of the age of the animal. Each point represents one brain slice. Loading is close to 100% at P10, but it declines to ≈70% of the neurons at P20. The line represents the best linear fit.
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Loading mature cortical slices with <t>fura-2</t> AM. DIC (A) and fluorescence (B) images of the lower layers of a visual cortex slice from a P10 mouse with the pial surface to the bottom right. The slice was loaded with fura-2 AM. Somata and apical dendrites from many neurons are fluorescent, and pyramidal and nonpyramidal neurons label equally. (Scale bar = 20 μm.) (C) Percentage of loaded neurons as a function of the age of the animal. Each point represents one brain slice. Loading is close to 100% at P10, but it declines to ≈70% of the neurons at P20. The line represents the best linear fit.
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Loading mature cortical slices with <t>fura-2</t> AM. DIC (A) and fluorescence (B) images of the lower layers of a visual cortex slice from a P10 mouse with the pial surface to the bottom right. The slice was loaded with fura-2 AM. Somata and apical dendrites from many neurons are fluorescent, and pyramidal and nonpyramidal neurons label equally. (Scale bar = 20 μm.) (C) Percentage of loaded neurons as a function of the age of the animal. Each point represents one brain slice. Loading is close to 100% at P10, but it declines to ≈70% of the neurons at P20. The line represents the best linear fit.
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Loading mature cortical slices with <t>fura-2</t> AM. DIC (A) and fluorescence (B) images of the lower layers of a visual cortex slice from a P10 mouse with the pial surface to the bottom right. The slice was loaded with fura-2 AM. Somata and apical dendrites from many neurons are fluorescent, and pyramidal and nonpyramidal neurons label equally. (Scale bar = 20 μm.) (C) Percentage of loaded neurons as a function of the age of the animal. Each point represents one brain slice. Loading is close to 100% at P10, but it declines to ≈70% of the neurons at P20. The line represents the best linear fit.
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Image Search Results


Loading mature cortical slices with fura-2 AM. DIC (A) and fluorescence (B) images of the lower layers of a visual cortex slice from a P10 mouse with the pial surface to the bottom right. The slice was loaded with fura-2 AM. Somata and apical dendrites from many neurons are fluorescent, and pyramidal and nonpyramidal neurons label equally. (Scale bar = 20 μm.) (C) Percentage of loaded neurons as a function of the age of the animal. Each point represents one brain slice. Loading is close to 100% at P10, but it declines to ≈70% of the neurons at P20. The line represents the best linear fit.

Journal:

Article Title: Optical probing of neuronal circuits with calcium indicators

doi:

Figure Lengend Snippet: Loading mature cortical slices with fura-2 AM. DIC (A) and fluorescence (B) images of the lower layers of a visual cortex slice from a P10 mouse with the pial surface to the bottom right. The slice was loaded with fura-2 AM. Somata and apical dendrites from many neurons are fluorescent, and pyramidal and nonpyramidal neurons label equally. (Scale bar = 20 μm.) (C) Percentage of loaded neurons as a function of the age of the animal. Each point represents one brain slice. Loading is close to 100% at P10, but it declines to ≈70% of the neurons at P20. The line represents the best linear fit.

Article Snippet: We used a double-incubation protocol ( 11 , 12 ): ( i ) an initial incubation with 2–5 μl of a 1 mM fura-2 acetoxymethyl ester (AM) (Molecular Probes) in 100% DMSO solution for 2 min, and ( ii ) a second incubation in 3 ml of 10 μM fura-2 AM in ACSF for 60 min.

Techniques: Fluorescence, Slice Preparation